> Publications
Carbon source utilization patterns of Bacillus simplex ecotypes do not reflect their adaptation to ecologically divergent slopes in"Evolution Canyon", Israel. Sikorski, J., Pukall, R., Stackebrandt, E. (2008); FEMS Microbiology Ecology, in press
The temperature adaptive fatty acid content in Bacillus simplex strains from "Evolution Canyon", Israel. Sikorski, J., Brambilla, E., Kroppenstedt, R.M., Tindall, B.J. (2008); Microbiology, 154(8), 2416-2426
The original publication is available here.
Press release:
Bacteria Reveal Secret Of Adaptation At Evolution Canyon
Canyonwand als Labor der Evolution
Fettige Evolution
L’apparenza non inganna
Las bacterias se adaptan a las diferentes temperaturas alterando sus membranas celulares
Identifying the Fundamental Units of Bacterial Diversity:
A Paradigm Shift to Incorporate Ecology into Bacterial Systematics. Alexander Koeppel, Elizabeth B. Perry, Johannes Sikorski,
Danny Krizanc, Andrew Warner, David M. Ward, Alejandro P. Rooney, Evelyne Brambilla, Nora Connor, Eviatar Nevo, Rodney M. Ratcliff, and Frederick M. Cohan (2008);
Proc Natl Acad Sci USA, 105:2504-2509.
The original publication is available here.
The Ecotype Simulation software
Populations under microevolutive scruteny - what do we gain? Johannes Sikorski (2007); Archives of Microbiology, DOI:10.1007/s00203-007-0294-x. (author-created version), The original publication is available at (www.springerlink.com)
Patterns of thermal adaptation of Bacillus simplex to the microclimatically contrasting slopes of "Evolution Canyons" I and II, Israel. Sikorski, J. & Nevo, E.; Environmental Microbiology (2007) 9(3), 716–726 (doi:10.1111/j.1462-2920.2006.01193.x) (reprint request)
Identifying the Fundamental Units of Diversity Among Bacillus Isolates from ‘Evolution Canyon’ III Elizabeth B. Perry, Danny Krizanc, Alejandro P. Rooney, Johannes Sikorski, Eviatar Nevo, and Frederick M. Cohan ; Israel Journal of Ecology & Evolution (2006), 52, 543-5552 (reprint request)
Adaptive Mutations in RNA-based Regulatory Mechanisms: Computational and Experimental Investigations. Barash, D., Sikorski,J. Perry, E.B.,Nevo, E., Nudler, E.; Israel Journal of Ecology & Evolution (2006), 52, 263-279 (reprint request)
On the necessity to study natural bacterial populations - the model of Bacillus simplex from "Evolution Canyons" I and II, Israel. Sikorski, J. & Nevo, E.; Israel Journal of Ecology & (2006), 52, 527-542-279 (preprint) (Flyer to order the Symposium volume)
Adaptation and incipient sympatric speciation of Bacillus simplex under contrasting microclimatic stress at "Evolution Canyons" I and II, Israel. Sikorski, J. & Nevo, E. (2005). Proc Natl Acad Sci USA, 102 (44):15924-15929. (pdf) (SOM)
Genetic similarity relationships within the genus Pleurotus determined by RAPD analysis. Stajic, M., Sikorski, J., Wasser, SP & Nevo, E. (2005). Mycotaxon, 93, 247-255. (pdf)
Genomovars 11 to 18 of Pseudmonas stutzeri, isolated from soil and marine sediments. Sikorski, J., Lalucat, J. & Wackernagel, W. (2005). International Journal of Systematic and Evolutionary Microbiology, 55(5), 1767-1770. (pdf)
Identification of complex composition, strong strain diversity and directional selection in local Pseudmonas stutzeri populations from marine sediment and soils. Sikorski, J., Möhle, M. & Wackernagel, W. (2002a). Environ Microbiol 4, 465-476. (pdf)
Highly different levels of natural transformation are associated with genomic subgroups within a local population of Pseudmonas stutzeri from soil. Sikorski, J., Teschner, N. & Wackernagel, W. (2002b). Appl Environ Microbiol 68: 865-873. (pdf)
The structure of a local population of phytopathogenic Pseudomonas brassicacearum from agricultural soil indicates development under purifying selection pressure. Sikorski, J., Jahr, H. & Wackernagel, W. (2001a). Environ Microbiol 3, 176-86. (pdf)
Pseudomonas kilonensis sp. nov., a bacterium isolated from agricultural soil. Sikorski, J., Stackebrandt, E. & Wackernagel, W. (2001b). Int J Syst Evol Microbiol 51, 1549-55. (pdf)
The potential for intraspecific horizontal gene exchange by natural genetic transformation: sexual isolation among genomovars of Pseudmonas stutzeri. Lorenz, M. G. & Sikorski, J. (2000). Microbiology 146 Pt 12, 3081-90.
(pdf)
This paper was chosen for the section of "Hot off the Press" of the journal Microbiology Today from the Society for General Microbiology (Vol. 28, 2001).
(pdf)
Analysis of genotypic diversity and relationships among Pseudmonas stutzeri strains by PCR-based genomic fingerprinting and multilocus enzyme electrophoresis. Sikorski, J., Rossello-Mora, R. & Lorenz, M. G. (1999). Syst Appl Microbiol 22, 393-402. (pdf)
Natural genetic transformation of Pseudmonas stutzeri in a non-sterile soil. Sikorski, J., Graupner, S., Lorenz, M. G. & Wackernagel, W. (1998). Microbiology. 144, 569-576. (pdf)
>Book chapters
Phenotypic Characterization and the Principles of Comparative Systematics. Brian J. Tindall, Johannes Sikorski, Robert A. Smibert, and Noel R. Krieg. Methods for General and Molecular Microbiology, 3rd Edition. Editors: C. A. Reddy, Terry J. Beveridge, John A. Breznak, George Marzluf, Thomas M. Schmidt, Loren R. Snyder. Book ISBN or Item Number: 978-1-55581-223-2.
Stability of recombinant DNA in soil and of its transforming potential for bacteria. Wackernagel, W., de Vries, J., Sikorski, J., Lorenz, M.G. In: CCRO Workshop proceedings, 5.-6. März 1998, Noordwijkerhout (NL).
Natural genetic transformation in soil. Wackernagel, W., Sikorski, J., Blum, S., Lorenz, M.G, Graupner, S. In: M. Syvanen, C. Kado (eds.). Horizontal Gene Transfer - Implications and Consequences, Thomson Science & Professional, London (1998), p. 168-178. More
>Oral presentations
Bacillus simplex from “Evolution Canyon“, Israel: a natural model system to study bacterial adaptation, evolution, and speciation J. Sikorski; Spring meeting of the VAAM in Osnabrück (01.-04.4.2007)
The adaptational diversity of the bacterium Bacillus simplex from “Evolution Canyon“, Israel J. Sikorski; INTERNATIONAL CONFERENCE WORKSHOP “NEW HORIZONS IN EVOLUTIONARY BIOLOGY” Haifa, Israel (23.-25.01.2007)
Evolutionary dynamics of "Bacillus macroides" in "Evolution Canyon" I and II, Israel. J. Sikorski and Eviatar Nevo. Annual Meeting of the Israli Society for Microbiology, Tel Aviv (2. 2. 2005)
Adaptation and incipient speciation of "Bacillus macroides" due to microclimatic stress in "Evolution Canyons" I and II, Israel. J. Sikorski and Eviatar Nevo. Oral presentation at the "10th International Symposium on Microbial Ecology - Microbial Planet: Sub-Surface to Space", Cancun, Mexico (22.-27. 08.2004)
Nonrandom distribution of RAPD genotypes of "Bacillus macroides" suggests incipient sympatric speciation due to microclimatic differences in "Evolution Canyon" I and II, Israel. J. Sikorski and E. Nevo; International Symposium on "Structure and Function of Soil Microbiota", Marburg, Germany (18.-20. 9. 2003)
Composition, structure, and transformability of local Pseudmonas stutzeri populations from soil and marine sediment. J. Sikorski and W. Wackernagel, Germany; 7th International Symposium on Bacterial Genetics and Ecology, Bergen, Norway (15.-19. 06. 2002)
Highly different levels of autologous and heterologous natural transformation among and between genomic subgroups of a local population of Pseudmonas stutzeri from soil. J. Sikorski and W. Wackernagel, Germany; Spring meeting of the VAAM in Göttingen (24.-27.3.2002)
Microbial systematics: A review on the species concept and the classification of prokaryotic microorganisms. J. Sikorski; Annual meeting of the German Paleontology Society and the German Society on biological systematics in Oldenburg, Germany (17.-21.09.2001)
The contribution of in situ evolution and migration to the shape of local population structures of the eurytopic bacterium Pseudmonas stutzeri. J. Sikorski, N. Teschner and W. Wackernagel; Spring meeting of the VAAM in Oldenburg, Germany (25.-28.3.2001)
Analysis of four local populations of Pseudmonas stutzeri from soil and marine habitats: evidence for differential population development with respect to in situ evolution and migration. J. Sikorski, N. Teschner and W. Wackernagel; VAAM-Workshop on microbial systematics, population genetics and epidemiology, Berlin, Germany, 17./18. November 2000
Intraspecific sexual isolation in naturally transformable Pseudmonas stutzeri. Michael G. Lorenz and J. Sikorski; Spring meeting of the VAAM in München, Germany (12.-16.3.2000)
Distribution of natural transformability within environmental populations of Pseudmonas stutzeri. N. Teschner, J. Sikorski and W. Wackernagel; VAAM-Workshop on microbial systematics, population genetics and epidemiology, Berlin, Germany, 5./6. November 1999
Molecular genetic and phenotypic analysis of a soil bacterial population: differential divergence of members up to the level of species differentiation. J. Sikorski, M.G. Lorenz and W. Wackernagel; 6th International Symposium on Bacterial Genetics and Ecology, June 20 to 24, 1999, Florence, Italy
Diversification of a Pseudomonas corrugata-like population from a common ancestor. J. Sikorski, M.G. Lorenz and W. Wackernagel; Spring meeting of the VAAM in Göttingen, Germany (07.-10.3.1999)
Diversification in bacterial populations: Contribution to community development. J. Sikorski, W. Wackernagel and M. G. Lorenz; KÖRBER SYMPOSIUM on Molecular and Microsensor studies of microbial communities, 7-11 September 1998, Bremen, Germany
Development of competence and natural genetic transformation of Pseudmonas stutzeri in non-sterile soil. J. Sikorski, M.G. Lorenz and W. Wackernagel; Spring meeting of the VAAM in Hamburg, Germany (16.-19.3.1997)
** Natural genetic transformation of Pseudmonas stutzeri in non-sterile soil. J. Sikorski, M.G. Lorenz and W. Wackernagel; 5th International Symposium on Bacterial Genetics and Ecology, May 25 to 29, 1996, Nauplia, Greece
** This lecture was awarded with a prize for the best lecture of the young scientists.
>Poster papers
Adaptation and incipient speciation of "Bacillus macroides" due to microclimatic stress in "Evolution Canyons" I and II, Israel. J. Sikorski and Eviatar Nevo. Poster presentation at the "10th International Symposium on Microbial Ecology - Microbial Planet: Sub-Surface to Space", Cancun, Mexico, August (22.-27. 08.2004)
Nonrandom distribution of RAPD genotypes of "Bacillus macroides" suggests incipient sympatric speciation due to microclimatic differences in "Evolution Canyon" I and II, Israel. J. Sikorski and E. Nevo; Annual Meeting of the Israeli Society of Microbiology, Haifa, Israel (9.-10. 9. 2004)
Genetic diversity of populations of Pseudmonas stutzeri. J. Sikorski, N. Teschner and W. Wackernagel. Spring meeting of the VAAM in München, Germany (12.-16.3.2000)
A highly diverse clonal population of phytopathogenic Pseudomonas corrugata in a denitrifying community of an agricultural field. J. Sikorski and M.G. Lorenz. Spring meeting of the VAAM in Frankfurt/M., Germany (22.-25.3.1998) Pseudmonas stutzeri is naturally transformable in non-sterile soil.
J. Sikorski, Lorenz, M.G. and W. Wackernagel (1996) Spring meeting of the VAAM in Bayreuth, Germany (March 1996)
